5 nov. 2009
Investigación del IMIBIO destacada en la revista internacional
La investigación realizada por las doctoras Susana Sánchez, María Arce, Lucía Fuentes y Gladys Ciuffo (IMIBIO-SL) será tapa de la revista internacional Experimental Neurology, en la edición de diciembre de 2009.
Prenatal blockade of Ang II receptors affects neonatal rat hindbrain structure and 2 receptor localization es el título del trabajo que será protagonista de esta revista que incluye trabajos de investigación relacionados a la neurobiología.
Resumen del trabajo en inglés (abstract)
The development of knock-out mice for Angiotensin II (Ang II) AT2 receptors, which exhibited altered 23 exploratory behavior, prompted us to investigate the cerebellum and brainstem. We evaluated the effect of 24 stimulation/inhibition of Ang II receptors on hindbrain development, in offspring (postnatal days P0, P8) of 25 pregnant rats treated during late pregnancy (Ang II, Losartan or PD123319, 1 mg/kg/day). Receptor 26 localization by autoradiography showed in P0 and P8 hindbrains, that most structures expressed AT2 27 subtype: cerebellar cortex, cerebellar nuclei, genu facial nucleus, inferior colicullus, inferior olive. In the 28 cerebellar cortex, [125I]Ang II AT2 binding was predominant, while low AT1 binding was observed in adjacent 29 layers of the cerebellar cortex. Blockade of AT2 receptors with PD123319 increased binding in cerebellar 30 nuclei (pb0.05) and brainstem nuclei at P0, P8, in correlation with increased AT2 receptor expression by RT- 31 PCR. The enlarged external granular layer (EGL) in PD123319-treated P0 pups contrast with the significant 32 decrease in Ang II binding (pb0.001) in the cerebellar cortex. Blockade of AT2 receptors during late 33 pregnancy seems to arrest cerebellar cortex development in P0 animals. On the contrary, increased AT2 34 binding was observed in cerebellar cortex and DTg nucleus in PD123319-treated P8 animals (pb0.001). Ang 35 II treatment leads to increased binding in the brainstem. In spite of the low doses of Ang II antagonists used, 36 treatments were performed during a time-frame critical for hindbrain development, leading to remarkable 37 effects. The present study makes a contribution to understand the role of Ang II receptors during hindbrain 38 development.
Más información en: Science Direct
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